Hi Maryalice! I have seen CD65 on a couple of ALL`s, both in children. Did you try CD79a - works nicely on paraffin with double APAAP! If positive - confirms ALL! Best wishes Anna Anna Porwit-MacDonald MD, PhD Haematopathology Lab., Karolinska Hospital Stockholm > >I recently had an interesting case. We received bone marrow on a 75 yo WF >with history of MDS. We noted immediately that she now has leukemia that is >+CD34, CD13, CD65 and CD10 but negative for CD33, CD71, CD11b, CD14, CD61, >CD41, CD42b, CD64, CD36, CD19, CD20, kappa, lambda, CD4, CD8, CD2, CD5, >CD16, CD56, and CD57. Unfortunately our MDS panel is set up to answer >protocol questions and doesn't have all leukemia antigens we would wish >(yes we have to worry about costs too). The blasts are weird, as expected >for MDS, and have scant cytoplasm, no granules and wild but small nuclei. >We then did some IP on the parrafin stuff and found the >following:myeloperoxidase -, Leu 22(CD43) + and Leu M1-CD15- weird spotty >+. TDT is pending. My question- Has any one seen CD65 on an ALL? What do >you think of this? > > > Maryalice > >Maryalice Stetler-Stevenson >Director Flow Cytometry Unit >Laboratory of Pathology, NCI, NIH > > > >
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