I am not familiar with CD65 per se, but I do not think a T-lymphoblastic process has been excluded. They often show coexpression of myeloid antigens. I do not see that either a CD7 or CD3 has been performed. Of course it could be a myeloid leukemia, I just do not think it has been proven as of yet. Any cytogenetic abnormalities to help us out? Karen Mann Duke University Medical Center _______________________________________________________________________________ Subject: Re: Interesting case From: melkhali@hotmail.com at internet Date: 12/03/97 8:41 AM The case you have described is most likely AML (CD13+, CD65+). Aberrant expression of lymphoid antigens is frequently seen in MDS and CML evolving into acute myelogenous leukemia. Less frequently, we have also seen dim expression of CD10. Normally, CD10 is very dimly expressed by mature neutrophils. Isolated CD10 expression should not, by its self, suggest lymphocytic differentiation. >Date: Mon, 1 Dec 1997 16:09:54 -0400 >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >From: stetler@box-s.nih.gov (Maryalice Stetler-Stevenson) >Subject: Interesting case > > >I recently had an interesting case. We received bone marrow on a 75 yo WF >with history of MDS. We noted immediately that she now has leukemia that is >+CD34, CD13, CD65 and CD10 but negative for CD33, CD71, CD11b, CD14, CD61, >CD41, CD42b, CD64, CD36, CD19, CD20, kappa, lambda, CD4, CD8, CD2, CD5, >CD16, CD56, and CD57. Unfortunately our MDS panel is set up to answer >protocol questions and doesn't have all leukemia antigens we would wish >(yes we have to worry about costs too). The blasts are weird, as expected >for MDS, and have scant cytoplasm, no granules and wild but small nuclei. >We then did some IP on the parrafin stuff and found the >following:myeloperoxidase -, Leu 22(CD43) + and Leu M1-CD15- weird spotty >+. TDT is pending. My question- Has any one seen CD65 on an ALL? What do >you think of this? > > > Maryalice > >Maryalice Stetler-Stevenson >Director Flow Cytometry Unit >Laboratory of Pathology, NCI, NIH > > > ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com RFC-822-headers: Received: from flowcyt.cyto.purdue.edu by mc.duke.edu (PMDF V5.1-10 #23776) with SMTP id <0EKM0040DN4BIV@mc.duke.edu>; Wed, 3 Dec 1997 13:53:08 -0500 (EST) Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) for cyto-sendout id KAA21125; Wed, 03 Dec 1997 10:30:01 -0500 Received: from hotmail.com by flowcyt.cyto.purdue.edu via SMTP (940816.SGI.8.6.9/930416.SGI.AUTO) for <cytometry@flowcyt.cyto.purdue.edu> id IAA20529; Wed, 03 Dec 1997 08:39:48 -0500 Received: (qmail 21284 invoked by uid 0); Wed, 03 Dec 1997 13:41:18 +0000 Received: from 195.128.0.144 by www.hotmail.com with HTTP; Wed, 03 Dec 1997 05:41:17 -0800 (PST) Date: Wed, 03 Dec 1997 05:41:17 -0800 (PST) From: mohamed elkhalifa <melkhali@hotmail.com> Subject: Re: Interesting case To: cyto-inbox Message-id: <19971203134118.21283.qmail@hotmail.com> Content-type: text/plain X-Originating-IP: [195.128.0.144]
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