Anna and Maryalice,
I think CD79a (and possibly c-kit) on paraffin is a good idea, but why
the assumption that this is ALL? I think that an M0 AML arising from
MDS (especially if there are background MDS changes) is more likely
than a CD19 negative ALL arising from MDS. While CD10 expression is
unusual in AML, we see it in 2.9% of our adult AML cases. Other than
the unusual CD10 expression, the immunophenotype does not appear to
exclude AML.
Dan Arber, M.D.
City of Hope
______________________________ Reply Separator _________________________________
Subject: Re: Interesting case
Author: anpo@mb.ks.se (Anna Porwit-MacDonald) at INTERNET
Date: 12/3/97 4:00 PM
Hi Maryalice!
I have seen CD65 on a couple of ALL`s, both in children.
Did you try CD79a - works nicely on paraffin with double APAAP!
If positive - confirms ALL!
Best wishes
Anna
Anna Porwit-MacDonald MD, PhD
Haematopathology Lab.,
Karolinska Hospital
Stockholm
>
>I recently had an interesting case. We received bone marrow on a 75 yo WF
>with history of MDS. We noted immediately that she now has leukemia that is
>+CD34, CD13, CD65 and CD10 but negative for CD33, CD71, CD11b, CD14, CD61,
>CD41, CD42b, CD64, CD36, CD19, CD20, kappa, lambda, CD4, CD8, CD2, CD5,
>CD16, CD56, and CD57. Unfortunately our MDS panel is set up to answer
>protocol questions and doesn't have all leukemia antigens we would wish
>(yes we have to worry about costs too). The blasts are weird, as expected
>for MDS, and have scant cytoplasm, no granules and wild but small nuclei.
>We then did some IP on the parrafin stuff and found the
>following:myeloperoxidase -, Leu 22(CD43) + and Leu M1-CD15- weird spotty
>+. TDT is pending. My question- Has any one seen CD65 on an ALL? What do
>you think of this?
>
>
> Maryalice
>
>Maryalice Stetler-Stevenson
>Director Flow Cytometry Unit
>Laboratory of Pathology, NCI, NIH
>
>
>
>
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