The case you have described is most likely AML (CD13+, CD65+). Aberrant expression of lymphoid antigens is frequently seen in MDS and CML evolving into acute myelogenous leukemia. Less frequently, we have also seen dim expression of CD10. Normally, CD10 is very dimly expressed by mature neutrophils. Isolated CD10 expression should not, by its self, suggest lymphocytic differentiation. >Date: Mon, 1 Dec 1997 16:09:54 -0400 >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >From: stetler@box-s.nih.gov (Maryalice Stetler-Stevenson) >Subject: Interesting case > > >I recently had an interesting case. We received bone marrow on a 75 yo WF >with history of MDS. We noted immediately that she now has leukemia that is >+CD34, CD13, CD65 and CD10 but negative for CD33, CD71, CD11b, CD14, CD61, >CD41, CD42b, CD64, CD36, CD19, CD20, kappa, lambda, CD4, CD8, CD2, CD5, >CD16, CD56, and CD57. Unfortunately our MDS panel is set up to answer >protocol questions and doesn't have all leukemia antigens we would wish >(yes we have to worry about costs too). The blasts are weird, as expected >for MDS, and have scant cytoplasm, no granules and wild but small nuclei. >We then did some IP on the parrafin stuff and found the >following:myeloperoxidase -, Leu 22(CD43) + and Leu M1-CD15- weird spotty >+. TDT is pending. My question- Has any one seen CD65 on an ALL? What do >you think of this? > > > Maryalice > >Maryalice Stetler-Stevenson >Director Flow Cytometry Unit >Laboratory of Pathology, NCI, NIH > > > ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
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