Home Seminal Contributions to Cytometry Wolfgang Göhde

Publications – Wolfgang Göhde

Greve B, Beller C, Cassens U, Sibrowski W, Göhde W. The impact of erythrocyte lysing procedures on the recovery of hematopoietic progenitor cells in flow cytometric analysis. Stem Cells. 2006 Mar;24(3):793-9.
Severin E, Greve B, Pascher E, Wedemeyer N, Hacker-Klom U, Silling G, Kienast J, Willich N, Göhde W. Evidence for predictive validity of blood assays to evaluate individual radiosensitivity. Int J Radiat Oncol Biol Phys. 2006 Jan 1;64(1):242-50.
Severin E, Kronholz HL, Kohnlein W, Göhde W. Dosimetry of soft x-rays in thin liquid layers. Phys Med Biol. 2005 Apr 7;50(7):1459-67.
Cassens U, Göhde W, Kuling G, Groning A, Schlenke P, Lehman LG, Traore Y, Servais J, Henin Y, Reichelt D, Greve B. Simplified volumetric flow cytometry allows feasible and accurate determination of CD4 T lymphocytes in immunodeficient patients worldwide. Antivir Ther. 2004 Jun;9(3):395-405.
Wedemeyer N, Pascher E, Cassens U, Göhde W. Quantification of PCR products using microparticles and flow cytometry. Ann Biol Clin (Paris). 2004 Jan-Feb;62(1):65-71.
Göhde W, Uthe D, Wedemeyer N, Severin E, Greif K, Schlegel D, Brede HJ, Kohnlein W. Mutagenic effect of low energy neutrons on human chromosome 11. Int J Radiat Biol. 2003 Nov;79(11):911-8.
Lizard G, Duvillard L, Wedemeyer N, Muller C, Ghiringhelli F, Cesbron A, Poncelet P, Gallet F, Kahn E, Gambert P, Göhde W. [Microbeads, nanobeads and cytometry: applications to the analysis and purification of cells and biomolecules] Pathol Biol (Paris). 2003 Sep;51(7):418-27.
Greve B, Beller C, Cassens U, Sibrowski W, Severin E, Göhde W. High-grade loss of leukocytes and hematopoietic progenitor cells caused by erythrocyte-lysing procedures for flow cytometric analyses. J Hematother Stem Cell Res. 2003 Jun;12(3):321-30.
Teodori L, Göhde W, Valente MG, Tagliaferri F, Coletti D, Perniconi B, Bergamaschi A, Cerella C, Ghibelli L. Static magnetic fields affect calcium fluxes and inhibit stress-induced apoptosis in human glioblastoma cells. Cytometry. 2002 Dec 1;49(4):143-9.
Wedemeyer N, Potter T, Wetzlich S, Göhde W. Flow cytometric quantification of competitive reverse transcription-PCR products. Clin Chem. 2002 Sep;48(9):1398-405.
Frankenberg-Schwager M, Garg I, Fran-Kenberg D, Greve B, Severin E, Uthe D, Göhde W. Mutagenicity of low-filtered 30 kVp X-rays, mammography X-rays and conventional X-rays in cultured mammalian cells. Int J Radiat Biol. 2002 Sep;78(9):781-9.
Frankenberg D, Frankenberg-Schwager M, Garg I, Pralle E, Uthe D, Greve B, Severin E, Göhde W. Mutation induction and neoplastic transformation in human and human-hamster hybrid cells
dependence on photon energy and modulation in the low-dose range. J Radiol Prot. 2002 Sep;22(3A):A17-20.
Cassens U, Greve B, Tapernon K, Nave B, Severin E, Sibrowski W, Göhde W. A novel true volumetric method for the determination of residual leucocytes in blood components. Vox Sang. 2002 May;82(4):198-206.
Potter T, Wedemeyer N, van Dulmen A, Kohnlein W, Göhde W. Identification of a deletion hotspot on distal mouse chromosome 4 by YAC fingerprinting. Mutat Res. 2001 May 9;476(1-2):29-42.
Wedemeyer N, Greve B, Uthe D, Potter T, Denklau D, Severin E, Hacker-Klom U, Kohnlein W, Göhde W. Frequency of CD59 mutations induced in human-hamster hybrid A(L) cells by low-dose X-irradiation. Mutat Res. 2001 Jan 25;473(1):73-84.
Janossy G, Jani I, Göhde W. Affordable CD4(+) T-cell counts on 'single-platform' flow cytometers I. Primary CD4 gating. Br J Haematol. 2000 Dec;111(4):1198-208.
Hacker-Klom UB, Kohnlein W, Göhde W. Effects of single and split doses of cobalt-60 gamma rays and 14 MeV neutrons on mouse stem cell spermatogonia. Radiat Res. 2000 Dec;154(6):667-72.
Wedemeyer N, Göhde W, Potter T. Flow cytometric analysis of reverse transcription-PCR products quantification of p21(WAF1/CIP1) and proliferating cell nuclear antigen mRNA. Clin Chem. 2000 Aug;46(8 Pt 1):1057-64.
Potter T, Göhde W, Wedemeyer N, Kohnlein W. Keratinocytes exposed to ultraviolet radiation reveal three down-regulated genes with potential function in differentiation and cell cycle control. Radiat Res. 2000 Aug;154(2):151-8.
Hacker-Klom UB, Kohnlein W, Kronholz HL, Göhde W. The relative biological effectiveness of low doses of 14 MeV neutrons in steady-state murine spermatogenesis as determined by flow cytometry. Radiat Res. 2000 Jun;153(6):734-42.
Tagliaferri F, Teodori L, Valente MG, Stipa F, Cucina A, Göhde W, Colettii D, Alo P, Stipa S. In vitro proliferation and in vivo malignancy of cell lines simultaneously derived from a chemically-induced heterogeneous rat mammary tumor. In Vitro Cell Dev Biol Anim. 2000 Mar;36(3):163-6.
Teodori L, Tagliaferri F, Stipa F, Valente MG, Coletti D, Manganelli A, Guglielmi M, D'Angelo LS, Schafer H, Göhde W. Selection, establishment and characterization of cell lines derived from a chemically-induced rat mammary heterogeneous tumor, by flow cytometry, transmission electron microscopy, and immunohistochemistry. In Vitro Cell Dev Biol Anim. 2000 Mar;36(3):153-62.
Hacker-Klom UB, Göhde W, Nieschlag E, Behre HM. DNA flow cytometry of human semen. Hum Reprod. 1999 Oct;14(10):2506-12.
Teodori L, Göhde W, Persiani M, Ferrario F, Tirindelli Danesi D, Scarpignato C, Di Tondo U, Alo P, Capurso L. DNA/protein flow cytometry as a predictive marker of malignancy in dysplasia-free Barrett's esophagus: thirteen-year follow-up study on a cohort of patients. Cytometry. 1998 Dec 15;34(6):257-63.
Teodori L, Persiani M, Cicconetti F, Di Tondo U, Alo P, Marci A, Brun S, Göhde W. Increased yield of cancer cell detection in bronchial washing fluid combining conventional cytology and high-resolution flow cytometry. Eur J Histochem. 1997;41 Suppl 2:145-6.
Cicconetti F, Teodori L, Persiani M, Di Tondo U, Alo P, Marci A, Brun S, Göhde W. Increased number of cancer cells in bronchial washing fluid detected by combining conventional cytology and high-resolution flow cytometry. J Cancer Res Clin Oncol. 1997;123(10):571-7.
Dolezel J, Göhde W. Sex determination in dioecious plants Melandrium album and M. rubrum using high-resolution flow cytometry. Cytometry. 1995 Feb 1;19(2):103-6.
Salvati F, Teodori L, Trinca ML, Pasquali-Lasagni R, Göhde W. The relevance of flow-cytometric DNA content in the evaluation of lung cancer. J Cancer Res Clin Oncol. 1994;120(4):233-9.
Teodori L, Trinca ML, Salvati F, Berettoni L, Storniello G, Göhde W. Cellular heterogeneity of DNA/total-protein content in human lung tumors, as determined by flow cytometry. Int J Cancer. 1992 Apr 1;50(6):845-53.
Jiao L, Gray DW, Göhde W, Flynn GJ, Morris PJ. In vitro staining of islets of Langerhans for fluorescence-activated cell sorting. Transplantation. 1991 Sep;52(3):450-2.
Heiden T, Schumann J, Göhde W. Flow sorting of tumor cells for morphometric analysis, particularly of rare cells. Virchows Arch B Cell Pathol Incl Mol Pathol. 1991;61(1):29-38.
Heiden T, Göhde W, Tribukait B. Two-wavelength mercury arc lamp excitation for flow cytometric DNA-protein analyses. Anticancer Res. 1990 Nov-Dec;10(6):1555-62.
Jager S, Menger MD, Göhde W, Themann H. Transplantation of islets of Langerhans in millipore diffusion chambers. Transplant Proc. 1990 Apr;22(2):870.
Jager S, Menger MD, Göhde W, Feifel G. A specific fluorescent dye for ex situ staining of vital islets of Langerhans: neutral red. Eur Surg Res. 1990;22(1):8-13.
Brauneis JW, Laskawi R, Schroder M, Göhde W. [Results of impulse cytophotometry in malignant tumors of the head and neck. A prognostic parameter?] HNO. 1989 Sep;37(9):369-72.
Laskawi R, Brauneis J, Schroder M, Göhde W. [The prognostic value of flow cytometry in malignancies of the head and neck] Onkologie. 1989 Aug;12(4):190-1.
Wagner W, Göhde W, Harle A, Bottcher HD. Histologic changes, proliferation kinetics, and growth patterns in squamous carcinomas and bone tumors transplanted to nude mice. Strahlenther Onkol. 1989 Jul;165(7):487-8.
Wagner W, Bottcher HD, Göhde W, Harle A. Radiosensitivity of osteosarcomas on nude mice. A comparative study of treatment with fast neutrons and cobalt 60. Strahlenther Onkol. 1989 Jul;165(7):515-6.
Hacker-Klom U, Heiden T, Otto FJ, Mauro F, Göhde W. Radiation-induced diploid spermatids in mice. Int J Radiat Biol. 1989 May;55(5):797-806.
Gray DW, Göhde W, Carter N, Heiden T, Morris PJ. Separation of pancreatic islets by fluorescence-activated sorting. Diabetes. 1989 Jan;38 Suppl 1:133-5.
Langer EM, Hemmer J, Kleinhans G, Göhde W. The applications of the BrdUrd-technique for the estimation of cycling S-phase cells in human renal cell carcinoma. Urol Res. 1988;16(4):303-7.
Franchi F, Seminara P, Codacci-Pisanelli G, Familiari M, Teodori L, Göhde W. A new anthracycline regimen for prolymphocytic leukemia? Study of a case report with flow cytometric implications. Leuk Res. 1987;11(10):947-9.
Kleinhans G, Hacker-Klom U, Göhde W, Korner F, Schumann J. [Studies of the cell kinetics of human malignant testicular tumors] Urologe A. 1986 Sep;25(5):294-7.
Hiddemann W, Wormann B, Göhde W, Buchner T. DNA aneuploidies in adult patients with acute myeloid leukemia. Incidence and relation to patient characteristics and morphologic subtypes. Cancer. 1986 Jun 1;57(11):2146-52.
Mauro F, Teodori L, Schumann J, Göhde W. Flow cytometry as a tool for the prognostic assessment of human neoplasia. Int J Radiat Oncol Biol Phys. 1986 Apr;12(4):625-36.
Hacker-Klom UB, Meistrich ML, Göhde W. Effect of doxorubicin and 4'-epi-doxorubicin on mouse spermatogenesis. Mutat Res. 1986 Mar;160(1):39-46.
Mauro F, Göhde W, Schumann J, Teodori L, Spano M. Considerations in the design of possible cell cycle effective drugs. Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Feb;49(2):307-33. Review.
Hiddemann W, Wormann B, Ritter J, Thiel E, Göhde W, Lahme B, Henze G, Schellong G, Riehm H, Buchner T. Frequency and clinical significance of DNA aneuploidy in acute leukemia. Ann N Y Acad Sci. 1986;468:227-40.
Langer EM, Rottgers HR, Schliermann MG, Meier EM, Miltenburger HG, Schumann J, Göhde W. Cycling S-phase cells in animal and spontaneous tumours. I. Comparison of the BrdUrd and 3H-thymidine techniques and flow cytometry for the estimation of S-phase frequency. Acta Radiol Oncol. 1985 Nov-Dec;24(6):545-8.
Hacker-Klom U, Göhde W, Schumann J. Quantitative evaluation of spontaneous and radiation-induced polyploidisation processes in human and murine testes. Acta Radiol Oncol. 1985 Nov-Dec;24(6):503-7.
Hacker-Klom U, Meier EM, Göhde W. [Mammalian spermatogenesis as a biological indicator for ionizing radiation] Z Naturforsch [C]. 1985 Nov-Dec;40(11-12):898-907.
Kleinhans G, Hacker-Klom U, Göhde W, Korner F, Schumann J. [Measuring the DNA distribution pattern in human testicular tumors] Helv Chir Acta. 1985 Sep;52(3-4):425-8.
Hacker-Kolm U, Kleinhans G, Göhde W. Flow cytometric analysis of the maldescended testis. Andrologia. 1985 Jul-Aug;17(4):389-94.
Buchner T, Hiddemann W, Wormann B, Kleinemeier B, Schumann J, Göhde W, Ritter J, Muller KM, von Bassewitz DB, Roessner A, et al. Differential pattern of DNA-aneuploidy in human malignancies. Pathol Res Pract. 1985 Jan;179(3):310-7.
Spano M, Calugi A, Capuano V, de Vita R, Göhde W, Hacker-Klom U, Maistro A, Mauro F, Otto F, Pacchierotti F, et al. Flow cytometry and sizing for routine andrological analysis. Andrologia. 1984 Jul-Aug;16(4):367-75.
Barlogie B, Raber MN, Schumann J, Johnson TS, Drewinko B, Swartzendruber DE, Göhde W, Andreeff M, Freireich EJ. Flow cytometry in clinical cancer research. Cancer Res. 1983 Sep;43(9):3982-97.
Barranco SC, May JT, Boerwinkle W, Nichols S, Hokanson KM, Schumann J, Göhde W, Bryant J, Guseman LF. Enhanced cell killing through the use of cell kinetics-directed treatment schedules for two-drug combinations in vitro. Cancer Res. 1982 Jul;42(7):2894-8.
Hacker U, Schumann J, Göhde W. Mammalian spermatogenesis as a new system for biologic dosimetry of ionizing irradiation. Acta Radiol Oncol. 1982;21(5):349-51.
Otto FJ, Oldiges H, Göhde W, Jain VK. Flow cytometric measurement of nuclear DNA content variations as a potential in vivo mutagenicity test. Cytometry. 1981 Nov;2(3):189-91.
Muller RP, Addicks HW, Schumann J, Göhde W. [Cytokinetic and genetic characterization of squamous cell carcinomas in the head and neck area] Dtsch Zahnarztl Z. 1981 Nov;36(11):693-6.
Dosik GM, Barlogie B, White RA, Göhde W, Drewinko B. A rapid automated stathmokinetic method for determination of in vitro cell cycle transit times. Cell Tissue Kinet. 1981 Mar;14(2):121-34.
Hacker U, Schumann J, Göhde W, Muller K. Mammalian spermatogenesis as a biologic dosimeter for radiation. Acta Radiol Oncol. 1981;20(4):279-82.
Barlogie B, Drewinko B, Schumann J, Göhde W, Dosik G, Latreille J, Johnston DA, Freireich EJ. Cellular DNA content as a marker of neoplasia in man. Am J Med. 1980 Aug;69(2):195-203.
Dosik GM, Barlogie B, Göhde W, Johnston D, Tekell JL, Drewinko B. Flow cytometry of DNA content in human bone marrow: a critical reappraisal. Blood. 1980 May;55(5):734-40.
Hacker U, Schumann J, Göhde W. Effects of acute gamma-irradiation on spermatogenesis as revealed by flow cytometry. Acta Radiol Oncol. 1980;19(5):361-8.
Otto FJ, Oldiges H, Göhde W, Barlogie B, Schumann J. Flow cytogenetics of uncloned and cloned Chinese hamster cells. Cytogenet Cell Genet. 1980;27(1):52-6.
Bergerat JP, Barlogie B, Göhde W, Johnston DA, Drewinko B. In vitro cytokinetic response of human colon cancer cells to cis-dichlorodiammineplatinum(II). Cancer Res. 1979 Nov;39(11):4356-63.
Otto FJ, Hacker U, Zante J, Schumann J, Göhde W, Meistrich ML. Flow cytometry of human spermatozoa. Histochemistry. 1979 Jul 11;61(3):249-54.
Meistrich ML, Göhde W, White RA, Longtin JL. "Cytogenetic" studies of spermatids of mice carrying Cattanach's translocation by flow cytometry. Chromosoma. 1979;74(2):141-51.
Barlogie B, Göhde W, Drewinko B. Flow cytometric analysis of DNA content for ploidy determination in human solid tumors. J Histochem Cytochem. 1979 Jan;27(1):505-7.
Göhde W, Meistrich M, Meyn R, Schumann J, Johnston D, Barlogie B. Cell-cycle phase-dependence of drug-induced cycle progression delay. J Histochem Cytochem. 1979 Jan;27(1):470-3.
Barlogie B, Göhde W, Johnston DA, Smallwood L, Schumann J, Drewinko B, Freireich EJ. Determination of ploidy and proliferative characteristics of human solid tumors by pulse cytophotometry. Cancer Res. 1978 Oct;38(10):3333-9.
Meistrich ML, Göhde W, White RA, Schumann J. Resolution of X and Y spermatids by pulse cytophotometry. Nature. 1978 Aug 24;274(5673):821-3.
Göhde W, Schumann J. [The combination of noxa for cell inactivation] Strahlentherapie [Sonderb]. 1978;75:34-7.
Schumann J, Göhde W. [Experimental studies on the inactivation of tumor cells with a combination of chemo- and radiation therapy] Strahlentherapie [Sonderb]. 1978;75:27-33.
Zante J, Schumann J, Göhde W, Hacker U. DNA-fluorometry of mammalian sperm. Histochemistry. 1977 Oct 3;54(1):1-7.
Barlogie B, Drewinko B, Göhde W, Bodey GP. Lethal and kinetic effects of peptichemio on cultured human lymphoma cells. Cancer Res. 1977 Aug;37(8 Pt 1):2583-8.
Van Dilla MA, Gledhill BL, Lake S, Dean PN, Gray JW, Kachel V, Barlogie B, Göhde W. Measurement of mammalian sperm deoxyribonucleic acid by flow cytometry. Problems and approaches. J Histochem Cytochem. 1977 Jul;25(7):763-73.
Baisch H, Göhde W, Linden WA. Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle. Radiat Environ Biophys. 1975 Jun 13;12(1):31-9.
Schumann J, Ehring F, Göhde W. Cell synchronization in solid tumors. Recent Results Cancer Res. 1975;(52):206-14.
Buchner T, Barlogie B, Asseburg U, Hiddemann W, Kamanabroo D, Göhde W. Accumulation of S-phase cells in the bone marrow of patients with acute leukemia by cytosine arabinoside. Blut. 1974 Apr;28(4):299-300.
Schumann J, Göhde W. [Cytokinetic effect of bleomycin on mouse Ehrlich carcinoma in vivo. Possibilities of tumor cell synchronization for radiotherapy] Strahlentherapie. 1974 Mar;147(3):298-307.
Buchner T, Asseburg U, Kamanabroo D, Hiddemann W, Hiddemann RM, Barlogie B, Göhde W. [Clinical studies on combined chemotherapy of leukemia with partial cell synchronization] Verh Dtsch Ges Inn Med. 1974;80:1674-8.
Buchner T, Hiddemann W, Schneider R, Kamanabroo D, Göhde W. [Cell-kinetic effects of leukemia therapy in chemical medicine as based on the DNA-histogram with impulse cytophotometry] Med Welt. 1973 Oct 19;24(42):1616-7.
Göhde W, Dittrich W, Zinser KH, Prieshof J. [Impulse cytophotometric measurements on atypical cell smears from vagina and cervix uteri] Geburtshilfe Frauenheilkd. 1972 May;32(5):382-93.
Buchner T, Göhde W, Dittrich W, Barlogie B. [Proliferation kinetics of leukemias before and during therapy as based on impulse cytophotometry] Verh Dtsch Ges Inn Med. 1972;78:159-62.
Reiffenstuhl G, Severin E, Dittrich W, Göhde W. [Impulse cytophotometry of vaginal and cervical smears] Arch Gynakol. 1971 Dec 31;211(4):595-616.
Göhde W, Dittrich W. [Cytostatic effect of daunomycin in impulsecytophotometric test] Arzneimittelforschung. 1971 Nov;21(11):1656-8.
Buchner T, Dittrich W, Göhde W. [Impulse-cytophotometry in hematologic cytology] Klin Wochenschr. 1971 Oct 1;49(19):1090-2.
Schumann J, Ehring F, Göhde W, Dittrich W. [Pulse cytophotometry of DNA in skin tumours] Arch Klin Exp Dermatol. 1971;239(4):377-89.
Buchner T, Dittrich W, Göhde W. [Impulse cytophotometry of blood cells and bone marrow cells] Verh Dtsch Ges Inn Med. 1971;77:416-8.
Dittrich W, Göhde W. [Impulse fluorometry of single cells in suspension] Z Naturforsch B. 1969 Mar;24(3):360-1.
Göhde W. [Use of flow cuvettes for the evaluation of density gradients in the preparative ultracentrifuge] Z Naturforsch B. 1969 Feb;24(2):250.
Göhde W, Berger M. [Alternating rates of increase in synchronized cultures of macrozoospores of the unicellular green alga Haematococcus pluvialis] Naturwissenschaften. 1966 Sep;53(18):482.