> from my understanding CD25 binds to IL-2R on T cells and CD122 binds > to IL-2R on NK cells. > > Are the molecules distinct genetically? > > Do both consist of the alpha, beta and gamma chains? > > Does one antibody interfere with the other? I mean, if I were to do > staining with 25 and 122, is it better to do them in distinct tubes? Maciej, The IL-2 receptor consists of three chains, alpha, beta and gamma and is the same on T cells and NK's. The gamma chain is shared by the receptors for IL-2, 4, 7, 9 and 15. CD25 is the alpha chain of the IL-2 receptor. This chain is not shared by any of the other T cell/NK growth factor receptors. CD122 is the beta chain of the IL-2 receptor. This chain is also used by the receptor for IL-15. These antibodies recognize different chains of the IL-2 receptor complex, and do not cross-block each other. Andrew D. Wells, Ph.D. University of Pennsylvania Department of Medicine 728 Clinical Research Building 415 Curie Boulevard Philadelphia, PA 19104 (215) 573-1840 [office] (215) 898-1951 [laboratory] (215) 573-2880 [FAX] adwells@mail.med.upenn.edu
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