Greetings, I have an ambitious postdoc who would like to analyze the protein content of exosomes by FACS. He tells me that exosomes range in size from 50 - 100 nM. Has anyone ever done this? or know if it is possible to do FACS analysis on "cells" this small? We have access to a FacScan, a FacScalibur, a FacsVantage and a MoFlo - any thoughts on which machine might be best for this analysis? Thanks for the input, Derek Sant'Angelo Derek Sant'Angelo, Ph.D. Assistant Member Immunology Program Memorial Sloan-Kettering Cancer Center 1275 York Ave. Box 492 New York, NY 10021 Phone: (212) 639-6894 Fax: (212) 794-4019 Email: d-sant'angelo@ski.mskcc.org
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