Hi Derek, we have used FITC for labelling and measurement protein content
in very small cells, like leishmania and E. Coli. In order to do that
measurement, we acquire the cells with log FSC and Log SSC. We set up the
gate on FSC and SSC and do histograms for FL1. It is very simple and is a
routine assay for our parasitology partners. Our paper describing this
measurement will come in Cytometry-August. In regard to equipment, a well
calibrated FacsCalibur can do the job, I hope this information will help
your "ambitious postdoc". Please contact me if you need more detailed
protocols.
Regards Rafael
>Greetings,
>
>I have an ambitious postdoc who would like to analyze the protein content
>of exosomes by FACS. He tells me that exosomes range in size from 50 - 100
>nM.
>
>Has anyone ever done this? or know if it is possible to do FACS analysis on
>"cells" this small?
>
>We have access to a FacScan, a FacScalibur, a FacsVantage and a MoFlo - any
>thoughts on which machine might be best for this analysis?
>
>Thanks for the input,
>
>Derek Sant'Angelo
>
>Derek Sant'Angelo, Ph.D.
>Assistant Member
>Immunology Program
>Memorial Sloan-Kettering Cancer Center
>1275 York Ave. Box 492
>New York, NY 10021
>Phone: (212) 639-6894
>Fax: (212) 794-4019
>Email: d-sant'angelo@ski.mskcc.org
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(o o)
________________________________oOo__(_)__oOo_________________________________
___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
/ o \/| | University Inst.for Virology http://www.vetvir.unizh.ch/
/ _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710
/_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911
______________________________________________________________________________
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