Dear friends, we would like to ask some advice r.e. the interpretation of some work we have been doing on induced sputum and BAL from normal subjects and COPD patients. We have been using 'flow' to look at the granulocytes, and examine the AV and PI staining in the events which we have gated on, which we feel are in the region in which one would expect to find granulocytes. Cells coming up AV+PI- we interpret as apoptotic; AV+PI+ as necrotic. This would seem to be conventionally how it is desribed in papers we have looked at. However, we were not sure how one might interpret cells which are AV-PI+? Are these just cells in an advanced state of necrosis? Is there another interpretation? We value any advice. With best wishes. yours sincerely, Martin Kelly P.S. since drafting this email, I have had a look through the excellent email archives, and found some commentary on this issue, but I don't think that the issue was ever really resolved. Any definitive opinions or references would therefore be appreciated. ---------------------- Martin Kelly Department of Clinical Biochemistry, Queen's University of Belfast, Institute of Clinical Science, Grosvenor Road, BELFAST. BT12 6BJ Tel No: +44 (0)28 9026 3267 Fax No: +44 (0)28 9023 6543 Email: m.g.kelly@qub.ac.uk
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