Thanks for your reply to our question. Yes, our understanding, too, is that AV+PI+ cells represent late apoptosis-early necrosis, and yes, we had interpreted AV-PI+ as later necrosis. We had been concerned, however, as there does appear to be some divergence of opinion. I will try to make the responses of people available if possible (by c.c.-ing my replies to the list) (there has already been good interest) - I hope this is an acceptable way of disseminating folk's responses. Thanks for your interest, and reply. Martin Kelly On Wed, 09 Feb 2000 18:17:41 -0500 Oscar J Trask <TRASK_OSCAR_J@Lilly.com> wrote: > > > Greetings Martin, > I am sure you will receive many messages on the interpretation. You are > right, cells that are AV+/PI- are apoptotic (early apoptosis), however, cells > that are AV+/PI+ may also be apoptotic and should be reported as late > apoptosis/early necrosis. AV-/PI+ are cells in necrosis. I would be > interested in hearing your responses. > Regards, > Joe Trask > > > > > > Martin Kelly <M.G.Kelly@queens-belfast.ac.uk> on 02/09/2000 07:29:49 AM > > > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > cc: > > Subject: Annexin V (AV) and propidium iodide (PI) staining to identify > apoptotic/necrotic cells > > > > > Dear friends, > we would like to ask some advice r.e. the interpretation of some > work we have been doing on induced sputum and BAL from normal > subjects and COPD patients. We have been using 'flow' to look at > the granulocytes, and examine the AV and PI staining in the > events which we have gated on, which we feel are in the region > in which one would expect to find granulocytes. Cells coming up > AV+PI- we interpret as apoptotic; AV+PI+ as necrotic. This would > seem to be conventionally how it is desribed in papers we have > looked at. However, we were not sure how one might interpret > cells which are AV-PI+? Are these just cells in an advanced > state of necrosis? Is there another interpretation? > We value any advice. > With best wishes. yours sincerely, > > Martin Kelly > > P.S. since drafting this email, I have had a look through the > excellent email archives, and found some commentary on this > issue, but I don't think that the issue was ever really > resolved. Any definitive opinions or references would therefore > be appreciated. > > ---------------------- > Martin Kelly > Department of Clinical Biochemistry, > Queen's University of Belfast, > Institute of Clinical Science, > Grosvenor Road, > BELFAST. BT12 6BJ > Tel No: +44 (0)28 9026 3267 > Fax No: +44 (0)28 9023 6543 > Email: m.g.kelly@qub.ac.uk > > > > > > > > ---------------------- Martin Kelly Department of Clinical Biochemistry, Institute of Clinical Science, Grosvenor Road, BELFAST. BT12 6BJ Tel No: (01232) 263267
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