Carol, thanks for your reply. Yes, we had worried that this might be so. However, when we examined results from controls and COPD in BAL, and found that the AV-PI+ rates were different. If it was the case that this was simply due to, for example the BAL or procedure, or my processing of the sample, would one not expect that the AV-PI+ rates would be similar? Martin Kelly On Thu, 10 Feb 2000 10:21:17 -0500 Carol.W.Johnson@ap.pnu.com wrote: > > > I think AV-PI+ are cells that were killed with during the procedure. > > Carol > > > > > Martin Kelly <M.G.Kelly@queens-belfast.ac.uk> on 02/09/2000 07:29:49 AM > > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > cc: (bcc: Carol W Johnson/USKZO/PNU) > Subject: Annexin V (AV) and propidium iodide (PI) staining to identify > apoptotic/necrotic cells > > > > > > Dear friends, > we would like to ask some advice r.e. the interpretation of some > work we have been doing on induced sputum and BAL from normal > subjects and COPD patients. We have been using 'flow' to look at > the granulocytes, and examine the AV and PI staining in the > events which we have gated on, which we feel are in the region > in which one would expect to find granulocytes. Cells coming up > AV+PI- we interpret as apoptotic; AV+PI+ as necrotic. This would > seem to be conventionally how it is desribed in papers we have > looked at. However, we were not sure how one might interpret > cells which are AV-PI+? Are these just cells in an advanced > state of necrosis? Is there another interpretation? > We value any advice. > With best wishes. yours sincerely, > > Martin Kelly > > P.S. since drafting this email, I have had a look through the > excellent email archives, and found some commentary on this > issue, but I don't think that the issue was ever really > resolved. Any definitive opinions or references would therefore > be appreciated. > > ---------------------- > Martin Kelly > Department of Clinical Biochemistry, > Queen's University of Belfast, > Institute of Clinical Science, > Grosvenor Road, > BELFAST. BT12 6BJ > Tel No: +44 (0)28 9026 3267 > Fax No: +44 (0)28 9023 6543 > Email: m.g.kelly@qub.ac.uk > > > > > > > > ---------------------- Martin Kelly Department of Clinical Biochemistry, Institute of Clinical Science, Grosvenor Road, BELFAST. BT12 6BJ Tel No: (01232) 263267
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