Thanks for your reply. The numbers are not usually too high (probably less than 10% total gated events) but is higher in some individuals, maybe moreso in normals, strangely. Someone else suggested cell nuclei (described as 'bare nuclei') also, as a possibility. My only contention with this is that one might not expect to find nuclei in the area on the FS v SS plot on which we have gated. We tend to gate in an area of high FS and SS, and i believe that nuclei wouldn't lie there. What do you think? Martin Kelly On Thu, 10 Feb 2000 10:13:57 -0800 "Dennis J. Young" <djyoung@ucsd.edu> wrote: > I vote for cell nuclei without PS-containing membranes. Never bothered to > sort them to verify this. Are there high numbers in your samples? > > > At 12:29 PM 2/9/00 +0000, you wrote: > > > >Dear friends, > >we would like to ask some advice r.e. the interpretation of some > >work we have been doing on induced sputum and BAL from normal > >subjects and COPD patients. We have been using 'flow' to look at > >the granulocytes, and examine the AV and PI staining in the > >events which we have gated on, which we feel are in the region > >in which one would expect to find granulocytes. Cells coming up > >AV+PI- we interpret as apoptotic; AV+PI+ as necrotic. This would > >seem to be conventionally how it is desribed in papers we have > >looked at. However, we were not sure how one might interpret > >cells which are AV-PI+? Are these just cells in an advanced > >state of necrosis? Is there another interpretation? > >We value any advice. > >With best wishes. yours sincerely, > > > > Martin Kelly > > > >P.S. since drafting this email, I have had a look through the > >excellent email archives, and found some commentary on this > >issue, but I don't think that the issue was ever really > >resolved. Any definitive opinions or references would therefore > >be appreciated. > > > >---------------------- > >Martin Kelly > >Department of Clinical Biochemistry, > >Queen's University of Belfast, > >Institute of Clinical Science, > >Grosvenor Road, > >BELFAST. BT12 6BJ > >Tel No: +44 (0)28 9026 3267 > >Fax No: +44 (0)28 9023 6543 > >Email: m.g.kelly@qub.ac.uk > > > > > > > > > > > > Dennis J. Young > Mail:<<mailto:djyoung@ucsd.edu>> > WWW:<<http://cancer.ucsd.edu/SResources/flow.htm>> > New Location & New Area Code, too! > (since June, 1999) Voice : (858) 822-0407 > Flow Cytometry Core Facility FAX : (858) 822-0403 > University of California, San Diego > Internal Medicine Group, Bldg #4, Rm 126 > 9500 Gilman Drive La Jolla CA 92093-0671 ---------------------- Martin Kelly Department of Clinical Biochemistry, Institute of Clinical Science, Grosvenor Road, BELFAST. BT12 6BJ Tel No: (01232) 263267
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