I am sorry, we seemed to have moved on from the original query. As I understood (misread?), the original query concerned the sub-G1 peak, whether this represented apoptotic cells and whether there could be necrotic cells present. It is now being suggested that small, highly fluorescent cells are being studied. What are these cells? How do you manage to observe a sub-G1 peak from PI staining by flow cytometry if you are precluded from making visual observation of the fluorescence? An alternative approach, involving slightly more work, is to fix and embed the cells for electron microscopy, cut a semi-thin section, stain with toluidene blue and to use conventional light microscopy (Ormerod MG, O'Neill CF, Robertson D, Harrap KR. Cisplatin induces apoptosis in a human ovarian carcinoma cell line without concomitant internucleosomal degradation of DNA. Exp. Cell Res. 1994; 206: 231-237). Alternatively, you could make a smear, visual the nuclei with a Giemsa or a Pap stain and again use conventional light microscopy. Michael Ormerod 34 Wray Park Road Reigate RH2 ODE Telephone: voice & FAX: (0)1737 241726 Mobile telephone: 0802 293242 Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
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