Jeffrey, At the risk of sounding grumpy, his best approach is to listen to you! The apoptosis experts have said over and over again that it is a mistake to rely upon one assay for apoptosis, and I believe a few recently pointed out the pitfalls of single-color PI on this list. I still suggest it for quick pilot studies, but I'm constantly after my users to back it up with other assays. Occasionally one will surprise me and take my advice. Steve (a core manager who often feels like his explanations fall upon deaf ears) On Thu, 9 Sep 1999, Jeffrey M Scott wrote: > > Fellow Flow-ers > I have an investigator looking at apoptosis by PI > alone. I have told him about TdT and Annexin V. What he is > wondering is how can one know that the sub-G0/G1 peak > ( which looks quite good ) is actually apoptosis and > if one assumes that it is; How can one know that those > events in the sub-G0/G1 peak are not necrotic. I have > talked with him at length about this topic and wondered > if anyone had any other ideas about how to address his > question > Jeffrey Scott > Baylor College of Medicine > Houston Texas > +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Steve G. Hilliard (706) 542-9474 University of Georgia Cell Analysis Facility flowman@uga.edu URL: http://floweb.cb.uga.edu/Floweb/ +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:58 EST