Fellow Flow-ers I have an investigator looking at apoptosis by PI alone. I have told him about TdT and Annexin V. What he is wondering is how can one know that the sub-G0/G1 peak ( which looks quite good ) is actually apoptosis and if one assumes that it is; How can one know that those events in the sub-G0/G1 peak are not necrotic. I have talked with him at length about this topic and wondered if anyone had any other ideas about how to address his question Jeffrey Scott Baylor College of Medicine Houston Texas
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