When asaying for apooptotic cells, you should always observe the nuclear morphology by light microscopy. The simplest way is to look at the PI stained nuclei using a fluorescent microscope. Alternative stains are Hoechst 33342 or acridine orange. The fragmented nuclei of apoptotic cells are generally quite characteristic Michael Ormerod 34 Wray Park Road Reigate RH2 ODE Telephone: voice & FAX: (0)1737 241726 Mobile telephone: 0802 293242 Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
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