Michael, considering that the cells you are looking at are very small and they show a natural fluorescence in all the wavelengths, have any ideas on how you could squash the natural and just pick the staining? Thanks Barb On Fri, 10 Sep 1999, Michael Ormerod wrote: > > When asaying for apooptotic cells, you should always observe the nuclear > morphology by light microscopy. The simplest way is to look at the PI > stained nuclei using a fluorescent microscope. Alternative stains are > Hoechst 33342 or acridine orange. The fragmented nuclei of apoptotic cells > are generally quite characteristic > > Michael Ormerod > 34 Wray Park Road > Reigate RH2 ODE > Telephone: voice & FAX: (0)1737 241726 > Mobile telephone: 0802 293242 > Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod >
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