Dear Marty, Many years ago, (about 1987), I was working on a graduate degree project that involved chromosome sorting. I was sorting CHO chromosomes with propidium iodide. I found this phenomenon as well. Granted, I was sorting on a Coulter 751 with a 5 Watt Argon laser, which considerably photobleached the PI, and I'm not sure what the smaller 15 mW lasers would do these days. When I would sort the chromosomes and then run them back through the cytometer, they would be much dimmer as a result of interaction with the laser. They also would fall out of the gate as you describe. Hope this helps. Cynthia B. Aller, Ph.D. Director Flow Cytometry Department Medical Science Laboratories 11020 W. Plank Court Wauwatosa, WI 53226 (414) 256-1480 caller@execpc.com
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