Hi, 1.Platelet adhesion is very commeon, especially if the sample is processed few hours after it is taken. If we get a very high positivity with CD61 or CD41 we perform cytoplasmic staining in BM slides or cytospins with APAAP method to see if the staining is specific. AML M7 are rare and I think that diagnosis should be confirm by this kind of staining. 2. We use similar procedure but we haven,t noticed such phenomenon. The only explanation I can figure out is that you loose cells while washing. Did you check other subpopulations (T cells?). Do they remain stable? Best wishes Anna At 18:57 1999-08-19 -1000, you wrote: > Two questions to toss out to the group: Looking for suggestions on >how to handle platelet adhesion to leukemic blasts when performing >immunophenotyping using CD41 and CD42b on whole blood/bone marrow >aspirates. Anyone have any ideas why? Anna Porwit-MacDonald Heamatopathology Lab. Department of Pathology Karolinska Hospital, Stockholm anpo@mb.ks.se tel.:+46-851775863 fax.:+46-851775843
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:53 EST