Two questions to toss out to the group: 1. Looking for suggestions on how to handle platelet adhesion to leukemic blasts when performing immunophenotyping using CD41 and CD42b on whole blood/bone marrow aspirates. 2. When doing surface kappa/lambda analysis on whole blood/bone marrow aspirates, we wash the cells prior to staining with a three color combination (CD19-Cy5/lambda-PE/kappa-FITC or CD20-Cy5/lambda-PE/kappa-FITC) to remove plasma and prevent false negative results. On some cases, we have noticed a much lower percentage of CD19 positive cells compared to results obtained on our initial screen, which is a stain/lyse/wash procedure using CD19-PE and CD20-PE or CD20-Cy5. Anyone have any ideas why?
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