Don, Our lab has done a bit of yeast work over the last few years without contamination problems of our mammalian cell cultures on the same sorter. We simply run 50 % bleach through our lines for about 30 min after the yeast sort (followed by DI water rinse). Phil Marder reference: L.J. Green, P. Marder, L.L. Mann, L-C Chio, W.L. Current. LY303366 exhibits rapid and potent fungicidal activity in flow cytometric assays of yeast viability. Antimicrob. Agents Chemother. 43: 830-835. 1999. --- "Walker, Don" <donwalker@chiroscience.com> wrote: > > Hi, > > I have a user that wants to start running yeast on the flow > cytometer. I have some trepidation about this. I have heard a > variety > of good, bad and ugly comments about this area. I know there are > some > experts in this area out there in FlowLand. My main concern is the > procedures needed afterwards to insure that no contamination occurs > in > subsequent sorts of mammalian cells. Do people who do yeast dedicate > an > instrument just for that? ( food and beverage industry )? One > thought, > without knowing how Herculean the cleaning efforts are, is to > dedicate a > separate sample line just for yeast. What chemicals, fungicides etc. > do > you yeast/flow experts out there use? Do you recommend periodic > cultures of the stream to test if the cleaning procedures are > adequate? > I'll share a short summary, with the list, of any responses I > receive. > TIA for your help with this. > > Sincerely, > Don Walker > > > __________________________________________________ Do You Yahoo!? Bid and sell for free at http://auctions.yahoo.com
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