Don, I think you are overly concerned. We routinely run yeast and bacteria, without problems to other samples. We normally clean our sample sipper with 10% bleach, and flush the sample delivery and sort collection areas with ethanol. Keep in mind that many applications looking at these beasties require fixation/permeabilization, as when looking at DNA. Also, unless you're sorting for cloning, most other applications will be fixable as well. MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry kukuru@umich.edu "Walker, Don" wrote: > Hi, > I have a user that wants to start running yeast on the flow > cytometer. I have some trepidation about this. I have heard a variety of > good, bad and ugly comments about this area. I know there are some > experts in this area out there in FlowLand. My main concern is the > procedures needed afterwards to insure that no contamination occurs in > subsequent sorts of mammalian cells. Do people who do yeast dedicate an > instrument just for that? ( food and beverage industry )? One thought, > without knowing how Herculean the cleaning efforts are, is to dedicate a > separate sample line just for yeast. What chemicals, fungicides etc. do > you yeast/flow experts out there use? Do you recommend periodic cultures > of the stream to test if the cleaning procedures are adequate? > I'll share a short summary, with the list, of any responses I receive. > TIA for your help with this. > Sincerely, > Don Walker
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