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From: Anja.Porwit@mb.ks.se
To: cyto-inbox
Subject: Re: Platelet adhesion to leukemic blasts
Date: 24 August 1999 22:19
Dercksen et al in Blood suggest flow cytometery in the presence of EDTA
which reduces the effect of P-selectin and integrins. I've found that total
removal from large volumes of PBMCs is rare, but is definitly aided by
washing with anti-coagulant (EDTA/acidfied citrate).
hope this is some help.
Craig
Hi,
1.Platelet adhesion is very commeon, especially if the sample is processed
few hours after it is taken. If we get a very high positivity with CD61 or
CD41 we perform cytoplasmic staining in BM slides or cytospins with APAAP
method to see if the staining is specific. AML M7 are rare and I think that
diagnosis should be confirm by this kind of staining.
2. We use similar procedure but we haven,t noticed such phenomenon. The
only explanation I can figure out is that you loose cells while washing.
Did you check other subpopulations (T cells?). Do they remain stable?
Best wishes
Anna
At 18:57 1999-08-19 -1000, you wrote:
> Two questions to toss out to the group: Looking for suggestions on
>how to handle platelet adhesion to leukemic blasts when performing
>immunophenotyping using CD41 and CD42b on whole blood/bone marrow
>aspirates. Anyone have any ideas why?
Anna Porwit-MacDonald
Heamatopathology Lab.
Department of Pathology
Karolinska Hospital, Stockholm
anpo@mb.ks.se
tel.:+46-851775863
fax.:+46-851775843
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