Greetings All: We are conducting a survey of PBL in transgenic mice. For the moment, we are looking at population groups and would subdivide the groups only if necessary. We using CD3e, CD45R/B220, and CD11b to examine T's,B's, monos-grans. Where our problem exists in in lysing the murine RBCs. We've been using ammonium chloride but the results have been very inconsistient and I was wondering if anyone has a "sure-fire" suggestion or recipe they'd be willing to share? Many thanks in advance, David ========================== David L. Haviland, PhD Assistant Professor, Immunology University of Texas, HSC - Houston Institute of Molecular Medicine 2121 W. Holcombe Blvd. Houston, TX 77030 http://www.uth.tmc.edu/~dhavilan 713.500.2413-Voice 713.500.2424-FAX ==========================
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