Dear Flow community:
Thanks for all of your responses to my inquiry. We ordered enough
screen from TWP Inc listed in Steve Lemoenic's very helpful response to
take care of our needs into the third millenium for less than $80.00.
For those of you who use frosted slides to prepare splenocytes, thanks
for the thought, but I am so fumble-fingered that I have not ever gotten
consistently uncontaminated splenocyte populations that way. With the
screens we make with long wire handles and using curved scissors to
mince the spleens, and 3 cc syringe plungers to press the chunks through
the screens, sterility is easy for us to maintain. Thanks again.
JHWIII
********************************************************
* John H. Wyckoff III,
PhD
*
* Associate Professor of
Immunology
*
* Department of Infectious Diseases and
Physiology *
* College of Veterinary
Medicine
*
* Oklahoma State
University
*
* Stillwater, Oklahoma
74078-2006
*
* telephone:
405-744-8176
*
* facsimile:
405-744-8263
*
* e-mail:
wyckoff@okstate.edu
*
********************************************************
\ | /
(o o)
_________________________oOo_ ( )__oOo________________
"I try to take one day at a time but lately several days
have attacked me at once!"
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