David,
We use Becton Dickinson FACS Lysing Solution. catalog # 92-0002
chris
Greetings All:
We are conducting a survey of PBL in transgenic mice. For the moment, we
are looking at population groups and would subdivide the groups only if
necessary. We using CD3e, CD45R/B220, and CD11b to examine T's,B's,
monos-grans.
Where our problem exists in in lysing the murine RBCs. We've been using
ammonium chloride but the results have been very inconsistient and I was
wondering if anyone has a "sure-fire" suggestion or recipe they'd be
willing to share?
Many thanks in advance,
David
==========================
David L. Haviland, PhD
Assistant Professor, Immunology
University of Texas, HSC - Houston
Institute of Molecular Medicine
2121 W. Holcombe Blvd.
Houston, TX 77030
http://www.uth.tmc.edu/~dhavilan
713.500.2413-Voice
713.500.2424-FAX
==========================
Chris Lena
Laboratory Technician
La Jolla Institue For Allergy and Immunology
phone:(619)678-4536
fax:(619)678-4595
cali@liai.org
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