20 micrometer-pore mesh

From: V Turcanu, Department of Pathology & Microbiology (Victor.Turcanu@bristol.ac.uk)
Date: Mon Jul 12 1999 - 06:51:29 EST


Dear All,

Does anybody know a commercial supplier of (approximately) 
20-micrometer pore mesh (or cells strainers or inserts for tissue 
culture) ?

I am asking because I want to do cell sorting of a cell population with 
10% of them larger then 20-micrometer. If I use a 100 micron nozzle, 
then the flow shall probably be turbulent because of the larger 
particles, so I would like to get rid of them. The mesh needs not to be 
exactly 20um but should be in this range (I saw 40um cell strainers 
from Falcon and 12 um pore cell culture inserts from Sigma but these 
are either too big or too small).

Thank you very much.

Victor Turcanu
Department of Pathology & Microbiology
University of Bristol, UK
Victor.Turcanu@bristol.ac.uk



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