Re: 20 micrometer-pore mesh

From: Heidi Engelhardt (hengel@APS.UoGuelph.CA)
Date: Mon Jul 12 1999 - 13:44:27 EST


Try:

SEFAR America Inc
4221 N.E. 34th Street
Kansas City, MO 64117
816-452-1520

They make "synthetic screening and filtration media" including Nitex nylon 
mesh.  According to the catalogue, they do have meshes in the 5-20 um 
range.  

Heidi

> Dear All,
> 
> Does anybody know a commercial supplier of (approximately) 
> 20-micrometer pore mesh (or cells strainers or inserts for tissue 
> culture) ?
> 
> I am asking because I want to do cell sorting of a cell population with
> 10% of them larger then 20-micrometer. If I use a 100 micron nozzle, then
> the flow shall probably be turbulent because of the larger particles, so I
> would like to get rid of them. The mesh needs not to be exactly 20um but
> should be in this range (I saw 40um cell strainers from Falcon and 12 um
> pore cell culture inserts from Sigma but these are either too big or too
> small).
> 
> Thank you very much.
> 
> Victor Turcanu
> Department of Pathology & Microbiology
> University of Bristol, UK
> Victor.Turcanu@bristol.ac.uk
> 


Dr. Heidi Engelhardt, Adjunct Professor
Department of Animal and Poultry Science
University of Guelph
Guelph, Ontario
CANADA N1G 2W1

phone:  519-824-4120, ext. 3658 or 8355
fax:    519-767-0573
email:  hengel@aps.uoguelph.ca
www:    http://www.aps.uoguelph.ca/faculty/heidi.html



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