Dear Gerard, We have analysed E. coli on our XL triggering on scatter (by the way, why not triggering on SSC? It uses to be more accurate than FSC on small particles). It is true that there are differences in scatter between different strains, but you should be able to distinguish E. coli from background with your cytometer (using log parameters). If not, you may consider recalibrating your instrument using small beads (1-2 microns)... Hope it helps, Jaume En/Na gerard ha escrit: > Dear Colleagues, > > We have a user trying to run E-coli cells that are either stained with > DiBac-4 or that are unstained. He wants to differentiate between the > populations using forward scatter. We're working with a Coulter Epics XL > at 488nm. His question is if it all is possible to run E-coli on an Epics > XL and if it's possible to differentiate between stained and unstained > cells. We're interested in any interesting comments or suggestions. > > Thanks in advance, > > Gerard Engelen-Eigles > Dept. of Agronomy and Plant Genetics > U. of Minnesota. -- Jaume Comas Citometria de Flux. Serveis CientíficoTècnics Universitat de Barcelona tel 34/93.402.13.46 fax 34/93.402.13.98 mailto:jaume@giga.sct.ub.es http://giga.sct.ub.es/personal/fitxes/jcr460.htm
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