Dear Victor, We are using woven nylon macroporous filter sheats "Spectra Mesh" that can be obtained in a variety of mesh sizes at "Spectrum", 1100 Rankin road, Houston, Texas, tel. 713 443 2900, fax 713 443 3100. I hope this can be of help. Best Regards, Dirk Prof. Dirk Van Bockstaele, PhD Laboratory of Hematology Antwerp University Hospital Wilrijkstraat 10 B-2650 Edegem Belgium tel. 32 3 821 3900, fax 32 3 825 1148, e-mail dirk.van.bockstaele@uza.uia.ac.be > ---------- > Van: V Turcanu, Department of Pathology & > Microbiology[SMTP:Victor.Turcanu@bristol.ac.uk] > Verzonden: maandag 12 juli 1999 13:51 > Aan: Cytometry Mailing List > Onderwerp: 20 micrometer-pore mesh > > > Dear All, > > Does anybody know a commercial supplier of (approximately) > 20-micrometer pore mesh (or cells strainers or inserts for tissue > culture) ? > > I am asking because I want to do cell sorting of a cell population with > 10% of them larger then 20-micrometer. If I use a 100 micron nozzle, > then the flow shall probably be turbulent because of the larger > particles, so I would like to get rid of them. The mesh needs not to be > exactly 20um but should be in this range (I saw 40um cell strainers > from Falcon and 12 um pore cell culture inserts from Sigma but these > are either too big or too small). > > Thank you very much. > > Victor Turcanu > Department of Pathology & Microbiology > University of Bristol, UK > Victor.Turcanu@bristol.ac.uk >
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