On Fri, 9 Jul 1999, Phil Marder wrote: > From: Phil Marder <mr_redram@yahoo.com> > > We can discern E. coli from noise on our XL's using LOG light scatter. Hi: Interesting! I once had a user that wanted to look at mitochondria and examine the changes in size during apoptosis with time. On the Calibur, with log FSC we were just at the edge outright machine noise but he was able to discern changes in the mitochondria. However, when I was at BD for training on the Vantage, I was told that getting a slightly larger FSC obscuration bar would give us the sensitivity that was needed to look at very small particles. Were you able to make such a change in the XL? Or did you need to? How far away were you from machine noise? I wonder if there may be a different FSC obscuration bar available for the Calibur as well? I too was told and heard about the Biorad machine but even from a former rep for BR, there was no logical reason why the machine was pulled from the market when it is clear there may be a niche for it. Many thanks, David ========================== David L. Haviland, PhD Assistant Professor, Immunology University of Texas, HSC - Houston Institute of Molecular Medicine 2121 W. Holcombe Blvd. Houston, TX 77030 http://www.uth.tmc.edu/~dhavilan 713.500.2413-Voice 713.500.2424-FAX ==========================
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