Colleagues, I'll ask the question again...it hasn't been answered here yet, twice. It wasn't answered in BD class when I asked it. It wasn't answered by the service engineers I've asked. Maybe no one has an answer sort of like that question in computer class years ago "does 'bit' derive from the first two letters of binary or the last two letters of digit?" We all understand that the Cy5 of the PE-Cy5 tandem can be excited by the red laser, that's the easy part. But if the signal generated from the two beams is separated in both space and time why should there be a compensation necessity? Furthermore, why on the FACSCalibur is it necessary to align the two beams in time when on the FACStar Plus the electronic separation of the two beams in time is considered a necessity? Gene Pizzo/UCONN Health
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