>Date: Sat, 29 Nov 1997 07:11:23 -0500 >From: Walter Sharp <denby@compuserve.com> >Subject: RE:APC/PE >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > > >Not that I know anything about the FacsCalibur, but just to add my >twopen'orth (cents) worth - > >Given that the delay between a cell passing through laser A and then laser >B is in the milliseconds range won't the fluorochromes excited in the first >beam still be fluorescing to a certain extent when they pass through the >second ? >I may be talking rubbish, but when doing fluorescence microscopy and you >turn the source off, the "glow" doesn't disappear immediately. >Maybe someone more into the physics of flow could answer this. > >Wal Sharp >"unemployed and bored" >UK Hi Wal, As far as I understand fluorescence occurs in the nano-second time frame, whereas phosphorecence occurs in the millisecond to second range. I think what you are seeing is the phosphorescence of the fluorochrome. Andy. Andy Riddell PNAC MRC-LMB Hills Road Cambridge UK tel (0) 1223 402218 fax (0) 1223 412178 email ar3@mrc-lmb.cam.ac.uk Andy Riddell PNAC MRC-LMB Hills Road Cambridge UK tel (0) 1223 402218 fax (0) 1223 412178 email ar3@mrc-lmb.cam.ac.uk
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