At 11:13 AM 26/11/97 -0500, Pizzo,Eugene wrote: >Colleagues, >I'll ask the question again...it hasn't been answered here yet, twice. >Maybe no one has an answer sort of like that question in computer class >years ago "does 'bit' derive from the first two letters of binary or the >last >two letters of digit?" As one lecturer put it, a bit is the smallest available piece, a byte is a mouthful of them, and I should not talk with my mouth full. >We all understand that the Cy5 of the PE-Cy5 tandem can be excited >by the red laser, that's the easy part. But if the signal generated from >the two beams is separated in both space and time why should there >be a compensation necessity? Without (correct) compensation, you don't know if the signal in FL4 is from APC or Cy5, or both. >Furthermore, why on the FACSCalibur is it necessary to align the two beams >in time when on the FACStar Plus the electronic separation of the two beams >in time is considered a necessity? Sorry, I don't understand the question... How are they different in principle? (yes, I know one is a sorter and the other is a sort of sorter, but..) -- -- Joseph Webster Flow Cytometry Facility Centenary Institute
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