Not that I know anything about the FacsCalibur, but just to add my twopen'orth (cents) worth - Given that the delay between a cell passing through laser A and then laser B is in the milliseconds range won't the fluorochromes excited in the first beam still be fluorescing to a certain extent when they pass through the second ? I may be talking rubbish, but when doing fluorescence microscopy and you turn the source off, the "glow" doesn't disappear immediately. Maybe someone more into the physics of flow could answer this. Wal Sharp "unemployed and bored" UK
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