lacZ kits/immunostaining of cytoplasmic proteins
kharkins@iastate.edu
Wed, 13 Oct 93 18:13:42 CDT
In reference to the lacZ question, my experience has been that the FDG or
lacZ kits are to be used with cells in the "viable" state, and that these
substrates are sensitive to the slightest perturbation of the cells which in
turn causes them to leak out. Does anyone know if the cleaved product readily
remains inside the cell once cleaved, should you then wish to "fix" the cells
for DNA/cell cycle staining? As Jeff stated, we are interested in using
beta-gal as a marker to distinguish one cell type from another within a mixed
culture, so that we can then in turn identify the individual cell cycle
distributions of each cell lineage.
Kristi Harkins
kharkins@iastate.edu
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