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On Wed, 13 Oct 1993 kharkins@iastate.edu wrote:
>
> In reference to the lacZ question, my experience has been that the FDG or
> lacZ kits are to be used with cells in the "viable" state, and that these
> substrates are sensitive to the slightest perturbation of the cells which in
> turn causes them to leak out. Does anyone know if the cleaved product readily
> remains inside the cell once cleaved, should you then wish to "fix" the cells
> for DNA/cell cycle staining? As Jeff stated, we are interested in using
> beta-gal as a marker to distinguish one cell type from another within a mixed
> culture, so that we can then in turn identify the individual cell cycle
> distributions of each cell lineage.
>
> Kristi Harkins
> kharkins@iastate.edu
>
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