RE: How many CD4/CD8 molecules per cell?
Dennis Broud 301-594-6259 FAX 301-443-9292 (BROUDD@cder.fda.gov)
Thu, 09 Nov 1995 08:31:08 -0400 (EDT)
Seems as I remember from my quantitative binding assay course that CD4's have
somewhere between 55,000 and 60,000 binding sites per cell. Depends on the
clone that you have, what epitope its directed against, the flurochrome you
label it with, etc. Check with the folks at the:
Center for Quantitative Cytometry
PO Box 12446
Research Triangle Park, NC 27709
Phone/Fax (919) 403-8229
They should be able to help you with all of your questions. Also the
have begun a database on specific clones and how many sites they recognize.
Good Luck
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
