Re: Old Parformaldehyde
Karenrt@aol.com
Tue, 7 Nov 1995 20:15:47 -0500
Electron Microscopy Sciences (New Jersey address; can get later if someone
needs it) sells a 16% solution of paraformaldehyde in 10 ml glass ampules.
Simply add to 150 ml of PBS and you have a 1% solution ready to go. It does
not need to be filtered and saves all the time needed to get the powder into
solution. Also, the powder can be considered toxic if breathed, so this
eliminates the safety hazard. The cost is relatively low and worth the time
savings. The 160 ml is usually used up quickly enough that storage (at room
temp - does not need fridge) and age are not a problem. The glass ampules are
a nuisance but there are ampule cutters on the market to simplify the chore.
Give it a try!
Karen R. Tamul, MS, MT(ASCP)SI
Technical Support Coordinator
FAST Systems, Inc
Gaithersburg, MD
(301) 977-0536
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
