 |
 |
 |
 |
> Hello, I have yet another question from the frozen expanses of Michigan
>
> We have a user that will be obtaining plueral and ascitic fluids from patients
> with malignant breast cancer that she would like to isolate the cells from and
> then run them on flow for DNA content/ apoptosis. She would like to know what
> would be the best way to draw this fluid ie, straight or with some anti-
> coagulant, how to store the fluid between the time that it is drawn and prepped,
> and any other tricks that others more experienced may be aware of.
>
> Thanks again for all the help
>
> Kris
>
We have had some success with breast cancer cells by aspirating the
tumour and storing the aspirate in cell culture freezing mixture, ie. 10%
DMSO in fetal calf serum. If I were trying the same with pleural
aspirates I would spin the fluid down to pellet the cells, re-suspend in
freezing mixtue and store at -80 C until I needed them. Vindelov's DNA
method works well after this, and end labelling for apoptotic cells can
also be made to work successfully after freezing mixture storage.
Hope this helps.
Eric, Edinburgh UK
|
|
|
|