prep and storage of plueral and ascitic fluid

kweber@vs4.im.med.umich.edu
Mon, 6 Nov 1995 15:07:17 -0500

Hello, I have yet another question from the frozen expanses of Michigan

We have a user that will be obtaining plueral and ascitic fluids from patients
with malignant breast cancer that she would like to isolate the cells from and
then run them on flow for DNA content/ apoptosis. She would like to know what
would be the best way to draw this fluid ie, straight or with some anti-
coagulant, how to store the fluid between the time that it is drawn and prepped,
and any other tricks that others more experienced may be aware of.

Thanks again for all the help

Kris

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