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A lot of the fears may simply be that, however a number of investigators I have
talked to make up fresh PFA for each use. I think the concern is old PFA causes
overfixation leading to increased nonspecific binding.
Considering how much trouble it is to make fresh PFA, we make up a couple of
litres at a time, aliquot them into 15 ml tubes and freeze the whole lot. I
will use thawed PFA for a week or two after thawing. I have heard that Ulf
Andersson's group in Stockholm use a similar approach, except they don't hold
on to the thawed PFA for 2 weeks.
Calman Prussin
Laboratory of Allergic Diseases
NIAID, NIH
---------------------- Replied Message Body ----------------------
Date: 11-2-95 6:48am
From: {herro001@maroon.tc.umn.edu}:unix:niaid
To: calman prussin:10:niaid,
karin hartmann:10:niaid,
barbara butcher:4:niaid,
ramya gopinath:4:niaid
Subj: Old Parformaldehyde
Also-to: cytometry mailing list <cytometry@flowcyt.cyto.purdue.edu>
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I have read the warnings to only use "fresh" parformaldehyde for fixation, but
I
don't recall ever hearing why.
Could someone explain the sorts of artifacts that old paraformaldehyde will
introduce? And what exactly IS old?
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