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Concerning your question about "anti mouse immunoglobulin
antibodies conjugated to a third colour, not anti mouse antibodies."
They have Tri-Color (an E transfer dye comprised of Cy5 coupled
to R-PE) reagents.
Give the company Caltag a try...their distributor is:
Cedarlane Laboratories Limited
5516-8th Line, R.R.2
Hornby, Ontario
Canada L0P 1E0
FAX: (905) 878-7800
Phone: (905) 878-8891
Hope this helps you.
D.Ladouceur
(613) 957-0998
______________________________ Reply Separator _________________________________
Subject: third colour indirect labeling
Author:
/G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com at
Internet
Date: 10/17/95 6:12 PM
Hi everybody
My question was actually regarding anti mouse immunoglobulin
antibodies conjugated to a third colour, not anti mouse
antibodies. Pharmingen do only some of their isotype
controls in cy-chrome. Using an epics-xl I actually want to
look at skin derived cells which have a high
autofluorescence. With the green autofluorescence I can
comfortably compensate the red autofluorescence down in the
first decade whilst I do not feel to good about doing it the
other way round and I would like to avoid having to use the
HENE on the epics elite.
Thanks to all who responded so far
Gerhard Nebe-v.Caron
Unilever Research, Colworth Laboratory Sharnbrook,
Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
E.mail: gerhard.nebe-von-caron@urcgb.sprint.com
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