Re: autofluorescence

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You might try autofluorescence compensation. Thjat technique has worked
well in our Facility on cultured cells with high autofluorescence. A
reference is enclosed.

-Marty Bigos
Stanford Shared FACS Facility

Alberti S; Bucci C; Fornaro M; Robotti A; Stella M.
Immunofluorescence analysis in flow cytometry: better selection of
antibody-labeled cells after fluorescence overcompensation in the red
channel.
Journal of Histochemistry and Cytochemistry, 1991 May, 39(5):701-6.

• Next message: PAUL@flowcyt.cyto.purdue.edu: "The ISAC abstract to end all abstracts...."
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• Maybe in reply to: Ton.Kiki: "autofluorescence"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu