MPO staining in whole blood

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Does anyone have experience with intracellular staining of whole blood using MPO
MoAbs. We are unable to overcome induction in MPO negative cells with samples
having high neutrophil counts. Have tried lots of permeabilising agents and
fixation strengths. Has anybody used 'Fix and Perm' from Caltag and does it
really work for MPO using whole blood methods.

Len Brown 102554.2147@compuserve.com

Department of Haematology (968)515736 voice
Sultan Qaboos University (968)513419 fax
Al Khod, Code 123, Sultanate of Oman

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu