Re: PE beads

Scott I. Simon (ssimon@bcm.tmc.edu)
Fri, 15 Sep 1995 17:37:42 -0500

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The use of quantum simply cellular beads will give a read out of the
relative fluorescence of any antibody-fluorchromeconjugate used without
the need for a calibration curve using beads which are fluoresceinated.
so either a FITC-IgG or a PE-IgG should still tell you the relative
number of antibody sites on the cells compared to the bead standard
(varying number of anti-IgG sites/bead). Does this partially anwser your
question?
Scott Simon
Baylor College of Medicine

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Maybe in reply to: Alice L. Givan: "PE beads"
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu