Re: PE beads
Howard Shapiro (hms@shapirolab.com)
Sat, 16 Sep 1995 14:58:46 -0400
In my experience, PE-conjugated beads don't hold up for more than a few
months, unlike FITC-beads, which have been very stable. Since many
PE-antibodies contain predominantly 1:1 conjugates of PE and immunoglobulin,
I have found it more reliable to use PE-antibody bound to capture beads for
standardization. If you get the PE-beads fresh, you can then run them with
dyed plastic beads to establish the dyed beads as a secondary standard,
although the correspondence will depend on you filter set and your PMT's
response curve and will only strictly hold for an individual instrument.
That may, however, be good enough for most purposes.
--Howard
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