Re: Texas Red Laser Excitation
Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Fri, 03 May 1996 08:01:32 +1100
At 04:53 PM 30/04/96 CDT, Kristi Harkins wrote: > We are looking into
laser configuration for a new cytometer and would like >to know if
anyone has found an alternative to the dye laser for excitation of
>Texas Red. I have noticed that the Coherent Spectrum laser puts out
about >150mW of 569nm. Would this be sufficient to excite Texas Red?
If you have
That should excite Texas Red, but it probably also excites PE. If you
wish to combine PE and TxR, there can be a crossover which cannot be
compensated out (on BD machines anyway, don't know for sure about
others.. Could some users of other machines comment?)
569 should also excite PI, which may or may not be useful. Is it too
low to excite APC?
Joseph. Joseph Webster (O.I.C. Flow Cytometry) Centenary
Institute of cancer Medicine & Cell Biology Locked Bag No.6, Newtown,
NSW 2042, AUSTRALIA. Ph: 61-2-565-6110 Fax: 61-2-565-6101
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
