Quantifying LIVE CELLS
Antony Bakke (bakkea@ohsu.EDU)
Fri, 15 Mar 96 08:43:25 PST
One option for measuring dead cells is using an enzyme substrate
like carboxy fluorescein diacetate succinimidyl ester. This is
cleaved by esters in the live cell to a form that is retained in the
cell for many hours. Dead cells lose their fluorescence quickly or
never gain any fluorescence if already dead when the subtrate is
added.
Tony Bakke
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
