Re: Quantifying LIVE CELLS

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I am planning to run a survival bioassay were then I can quantitate LIVE =
cells only using the flowcytometer.
I called Molecular Probes and they suggested "SYTOX" which stains dead =
cells only (but will not cross the membrane of live cells). Have anyone =
used this stain before? Advantages or Disadvantages?
or is there a live cell staining? Suggestions......

Osama Nabulsi
AMGEN INC.
(805)447-1594

Hi Osama,

Try using "Green Cell Tracker dye(CMFDA)" from Molecular Probes. This stains
viable cells at 1.0 uM concentrations. Staining is immediate with fluorescence
excitation at 490nm.

Good Luck,

Paul Hallberg
Retrovirology/HMJF

• Next message: aultk.mmcri@mmc.org: "Labeling Nuclear Antigens?"
• Previous message: Keith Bahjat: "Lymph Node Prep"
• Maybe in reply to: Antony Bakke: "Quantifying LIVE CELLS"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu